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| BORJA-OLIVEIRA,C. R.; SOARES,A. M.; ZAMUNÉR,S. R.; HYSLOP,S.; GIGLIO,J. R.; PRADO-FRANCESCHI,J.; RODRIGUES-SIMIONI,L.. |
| Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops neuwiedii venom; Intraspecific variation; Myotoxicity; Neurotoxicity; Neuromuscular blockade. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100008 |
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| Galbiatti,C; Rocha,T; Randazzo-Moura,P; Ponce-Soto,LA; Marangoni,S; Cruz-Höfling,MA; Rodrigues-Simioni,L. |
| Bmaj-9, a basic PLA2 (13679.33 Da), was isolated from Bothrops marajoensis snake venom through only one chromatographic step in reversed phase HPLC on ¼-Bondapak C-18 column. The amino acid composition showed that Bmaj-9 had a high content of Lys, His, and Arg, typical of a basic PLA2. The sequence of Bmaj-9 contains 124 amino acid residues with a pI value of 8.55, such as DLWQWGQMIL KETGKLPFSY YTAYGCYCGW GGRGGKPKAD TDRCCFVHDC, revealing a high homology with Asp49 PLA2 from other snake venoms. It also exhibited a pronounced phospholipase A2 activity when compared with crude venom. In chick biventer cervicis preparations, the time for 50% and 100% neuromuscular paralysis was respectively (in minutes): 110 ± 10 (1 µg/mL); 40 ± 6 and 90 ± 2 (5 µg/mL); 30 ± 3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops marajoensis; Snake venom; Asp49 PLA2; Neuromuscular blockade. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100008 |
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